Bouin’s Solution - 1.000 Ml - Sigma

Bouin′s solution

Sinônimo(s):

Bouin’s fluid

Número MDL:

MFCD00146169

NACRES:

NA.47

prazo de validade

Expiry date on the label

Nível de qualidade

200

composição

acetic acid, 5%
formaldehyde, 9%
picric acid, 0.9%

cor

yellow

application(s)

hematology
histology

temperatura de armazenamento

room temp

Descrição geral

Bouin′s solution is a noncoagulant yellow stain fixative that consists of saturated picric acid, formaldehyde, and acetic acid.

Aplicação

Bouin′s solution is an excellent fixative for preserving soft and delicate structures. It is used as a mordant in various trichrome procedures.
Additionally, it has been used for the following purposes-

staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging

fixation of rat ovaries for the observing follicle cells using microscopy

fixation of gonads of crab for morphological and histological analysis

Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Ações bioquímicas/fisiológicas

Bouin′s solution causes shrinkage of tissues when used as a preservative due to its picric acid component. It preserves morphological and nuclear information in tissues upon fixation.