Fluid Thioglycolate Medium Seg. Harm Ep/Usp/Jp - 500 Gr - Merck
For cultivation and isolation of obligate and facultative anaerobic and microaerophilic bacteria and for sterility tests.
Both culture media comply with the recommendations of United States Pharmacopeia XXVI (2003), the European Pharmacopeia and APHA (1992).
Mode of Action
The reducing agents thioglycollate and cystine ensure an anaerobiosis which is adequate even for fastidious anaerobes. The sulfhydryl groups of these substances also inactivate arsenic, mercury and other heavy metal compounds.
The thioglycollate media are thus suitable for the examination of materials which contain heavy metals or heavy metal preservatives. The higher viscosity of the Fluid Thioglycollate Medium prevents rapid uptake of oxygen. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes its colour to red.
Typical Composition (g/litre)
Peptone from casein 15.0;
yeast extract 5.0;
sodium chloride 2.5;
sodium thioglycollate 0.5;
sodium resazurin 0.001;
RESAZURINA Indicador de oxi-redução que forma anel de cor caeacterística na superfície do meio.