Middlebrook 7H11 Agar Base - 500 Gr - suitable for microbiology, NutriSelect^® Plus

Sinônimo(s):

Media for culturing Mycobacteria

esterilidade

non-sterile

Nível de qualidade

200

linha de produto

BioChemika

forma

powder

prazo de validade

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect^® Plus

condição de armazenamento

protect from light at room temperature

technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

pH final

6.6±0.2 (25 °C)

application(s)

clinical testing
food and beverages
life science and biopharma
veterinary
microbiology

adequação

mycobacteria

Descrição geral

Middlebrook 7H11 Agar Base media is used for the isolation, cultivation, and sensitivity testing of Mycobacterium species. This agar base contains many inorganic salts which help the growth of Mycobacteria. Citric acid formed from sodium citrate helps in retaining inorganic cations in solution. Glycerol supplies carbon and energy. The Middlebrook OADC Supplement (M0678) contains oleic acid, bovine albumin, sodium chloride, dextrose and catalase. Oleic acid and other long chain fatty acids are essential for the metabolism of Mycobacteria. Dextrose is an energy source. Catalase neutralizes toxic peroxides. Albumin protects tubercle bacilli from toxic agents. Malachite green partially inhibits other bacteria.

Aplicação

There are two types of solid culture media for the primary isolation of Mycobacteria. Egg-based (Lowenstein′s formulation) and agar-based (Middlebrook formulation)
The latter has the following advantages over the egg-based media

• The Middlebrook 7H11 agar base does not liquify easily in the presence of contaminating proteolytic organisms
• There is less inactivation of added drugs when egg ingredients are absent

Componentes

Ingredients (g/L)
Casein enzymatic hydrolysate, 1.00
Ammonium sulfate, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Magnesium sulfate, 0.05
L-Glutamic acid, 0.50
Ferric ammonium citrate, 0.04
Pyridoxine, 0.001
Biotin, 0.0005
Malachite green, 0.001
Agar, 15.00

Nota de preparo

Suspend 10.25 g of Middlebrook 7H11 Agar Base in 450 ml of distilled water. Add 2.5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add 1 vial Middlebrook OADC Growth Supplement (M0678). Mix thoroughly before dispensing.

Nota de rodapé

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informações legais

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

WGK

WGK 3

Ponto de fulgor (ºF)

Not applicable

Ponto de fulgor (ºC)

Not applicable