Synonym: β-DPNH, β-NADH, DPNH, Diphosphopyridine nucleotide, reduced form, NADH

• CAS Number 606-68-8 (anhydrous)

• Empirical Formula (Hill Notation) C₂₁H₂₇N₇Na₂O₁₄P₂ · xH₂O

• Molecular Weight 709.40 (anhydrous basis)

•  EC Number 210-123-3

•  MDL number MFCD03791273

•  PubChem Substance ID 329818676

Packaging

1, 5 g in poly bottle

50, 100, 500 mg in poly bottle

Packaged by solid weight.

Application

β-Nicotinamide adenine dinucleotide (NAD+) and β-Nicotinamide adenine dinucleotide, reduced (NADH) comprise a coenzyme redox pair (NAD+:NADH) involved in a wide range of enzyme catalyzed oxidation reduction reactions. In addition to its redox function, NAD+/NADH is a donor of ADP-ribose units in ADP-ribosylaton (ADP-ribosyltransferases; poly(ADP-ribose) polymerases ) reactions and a precursor of cyclic ADP-ribose (ADP-ribosyl cyclases).

As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.

Biochem/physiol Actions

NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD⁺/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease.
As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.