Albumina Sérica Bovina (BSA) Liofilizada 96% - 500 Gr - Sigma
Código: 15945
Bovine Serum Albumin lyophilized powder, ≥96% (agarose gel electrophoresis)
Sinônimo(s):
Albumin bovine serum, BSA, Bovine albumin
Número CAS:
Número EC:
Número MDL:
NACRES:
NA.27
fonte biológica
bovine
Nível de qualidade
teor
≥96% (agarose gel electrophoresis)
forma
lyophilized powder
peso molecular
~66 kDa
purificado por
cold ethanol fractionation
embalagem
poly bottle of
origem
USA origin
technique(s)
ELISA: suitable
blood typing: suitable
microbiological culture: suitable
perda
≤6%
pH
6.5-7.5 (1% in 0.15 M sodium chloride)
7
solubilidade
water: soluble (40 mg/ml)
nº de adesão UniProt
temperatura de armazenamento
2-8°C
Gene Information
bovine ... ALB(280717)
Bovine serum albumin has been used-
along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis[1]
as a component of modified Krebs-Ringer buffer (dmKRBT)[2]
in gel shift analysis[3]
Embalagem
10, 50, 100, 500 g in poly bottle
1, 5 kg in poly drum
Ações bioquímicas/fisiológicas
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Nota de preparo
Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
