Synonym: Coomassie dye binding protein assay, Protein dye reagent

Features and Benefits

• The reagent is ready to use. No mixing or dilution required.
• Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
• Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
• Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
• Can be used in microwell plate assays.
• Inexpensive assay.

Application

Bradford Reagent has been used to determine total protein concentration.^[1]

General description

Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.^[2]