2,2'-Azino-Bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) Liquid Substrate System - 100 Ml - Sigma
Código: 17216
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
Liquid Substrate System
MDL number MFCD00214141
PubChem Substance ID 24890730
NACRES NA.83
|
Quality Level |
|
|
form |
liquid |
|
storage temp. |
2-8°C |
|
SMILES string |
CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O |
|
InChI |
1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18- |
|
InChI key |
ZTOJFFHGPLIVKC-CLFAGFIQSA-N |
Descrição
General description
The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications.
Application
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product.[1][2] It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity.
Biochem/physiol Actions
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. The oxidized ABTS is a blue-green product and is measurable colorimetrically.[3] A combination of ABTS, hydrogen peroxide and myoglobin is a standard system employed for measuring total antioxidant capacity(TAC) from samples.[4] ABTS method is flexible at varying pH and most widely used.[5] ABTS is a substrate for phenol oxidase enzyme.[6]
Physical form
Ready-to-use.
