Fumarase From Porcine Heart - 500 UN - Sigma
Código: 16902
Fumarase from porcine heart
ammonium sulfate suspension, 300-500 units/mg protein (biuret)
Synonym: L-Malate hydrolyase, Fumarate hydratase
- CAS Number 9032-88-6
- Enzyme Commission (EC) Number 4.2.1.2 ( BRENDA | IUBMB )
- EC Number 232-887-7
- MDL number MFCD00131111
- NACRES NA.54
Propriedades
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Related Categories |
4.2.x.x C-O bonds, 4.x.x.x Lyases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index, Mais... |
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Quality Level |
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form |
ammonium sulfate suspension |
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foreign activity |
malic dehydrogenase ≤0.1% |
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storage temp. |
2-8°C |
Descrição
General description
Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.[2]
Fumarase is mapped to human chromosome 1q43.[3] Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.[4]
Application
Fumarase from porcine heart has been used:
• as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay[8]
• for immobilized enzyme based stability assay[9]
• in fumarate hydratase assays in human lung carcinoma cells (A549)[10]
Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.[1]
Biochem/physiol Actions
Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Fumarase is essential for regulation of cellular metabolism.[5] Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation.[6] Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome
