Thiazolyl Blue Tetrazolium Bromide 98%

Sinônimo(s):

MTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide

Empirical Formula (Hill Notation):

C₁₈H₁₆BrN₅S

Número CAS:

298-93-1

Peso molecular:

414.32

Beilstein:

4081397

Número EC:

206-069-5

Número MDL:

MFCD00011964

ID de substância PubChem:

24896603

NACRES:

NA.47

Nível de qualidade

200

teor

98%

forma

powder

pf

195 °C (dec.) (lit.)

solubilidade

2-methoxyethanol: soluble 20 mg/mL
ethanol: soluble 20 mg/mL
H₂O: 5 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

2-8°C

SMILES string

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

Aplicação

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Thiazolyl Blue Tetrazolium Bromide has been used for the following assays:

Proliferation assay.

TNF (tumor necrosis factor) bioassay.

Embalagem

1, 5, 10 g in glass bottle

100, 250 mg in glass insert

500 mg in glass bottle