N-Etilmaleimida (N-Ethylmaleimide) 98% - 25 Gr - Sigma
Código: 18074
N-Ethylmaleimide crystalline, ≥98% (HPLC)
Synonym: NEM
- CAS Number 128-53-0
- Empirical Formula (Hill Notation) C6H7NO2
- Molecular Weight 125.13
- Beilstein/REAXYS Number 112448
- EC Number 204-892-4
|
Quality Level |
|
|
biological source |
synthetic (organic) |
|
assay |
≥98% (HPLC) |
|
form |
crystalline |
|
bp |
210 °C (lit.) |
|
mp |
43-46 °C (lit.) |
|
solubility |
ethanol: 50 mg/mL, clear to slightly hazy, colorless to faint yellow or tan |
|
storage temp. |
2-8°C |
|
SMILES string |
CCN1C(=O)C=CC1=O |
|
InChI |
1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3 |
|
InChI key |
HDFGOPSGAURCEO-UHFFFAOYSA-N |
|
Gene Information |
human ... KCNQ2(3785), KCNQ4(9132), KCNQ5(56479), SLC6A3(6531), SLC6A4(6532) |
Descrição
Packaging
5, 25, 100 g in glass bottle
Application
Reagent for the covalent modification of cysteine residues in proteins.
Biochem/physiol Actions
Augments currents from native M-channels in sympathetic neurons and acts as an opener for KCNQ2, KCNQ4 and KCNQ5 channels.
Sulfhydryl alkylating agent that inactivates NADP-dependent isocitrate dehydrogenase and many endonucleases.
Preparation Note
NEM gives a clear solution in ethanol at 50 mg/ml. NEM dissolves in water (>50 mg in 4 ml); however, aqueous solutions are unstable. The rate of hydrolysis is pseudo-first order and significantly dependent on pH.
