Nile Red for microscopy

Sinônimo(s):

Nile Blue A Oxazone

Empirical Formula (Hill Notation):

C₂₀H₁₈N₂O₂

Número CAS:

7385-67-3

Peso molecular:

318.37

Beilstein:

279110

Número EC:

230-966-0

Número MDL:

MFCD00011639

ID de substância PubChem:

57652419

NACRES:

NA.32

grau

for microscopy

Nível de qualidade

100

forma

crystals

pf

203-205 °C (lit.)

λ_max

553 nm

SMILES string

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChI key

VOFUROIFQGPCGE-UHFFFAOYSA-N

Nile Red is a phenoxazone dye.[1] It is an important stain, which is used to detect intracellular lipid droplets[2] and proteins.[3] Nile Red detects lysosomes and lysosome-related organelles, like gut granules in C. elegans intestinal cells.[4] It exhibits strong fluorescence enhancement and is used for staining SDS (sodium dodecyl sulphate) gels.[1]

Aplicação

Nile Red has been used:

• as a fluorescent dye for staining fat by confocal scanning laser microscopy (CSLM)[5][6][7]

• to label nanoparticles (NPs)[8]

• for lipid analysis[9]