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B-Galactosidase From Aspergillus Oryzae 8.0 Units/Mg Solid - 500.000 Units - Sigma

Código: 19548

Compartilhe

B-Galactosidase From Aspergillus Oryzae 8.0 Units/Mg Solid - 25.000 Units - Sigma

Sinônimo(s):

β-D-Galactoside galactohydrolase, Lactase from Aspergillus oryzae

Número CAS:

9031-11-2

Número da licença da enzima:

3.2.1.23 (BRENDA, IUBMB)

Número EC:

232-864-1

Número MDL:

MFCD00130623

forma

solid

Nível de qualidade

200

specific activity

≥8.0 units/mg solid

contém

dextrin as extender

temperatura de armazenamento

−20°C

Descrição geral

β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.[1]

Aplicação

β-Galactosidase from Aspergillus oryzae has been used:

in in vitro nuclear magnet resonance (NMR)[2]

as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase[3]

to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties[4]

for enzymatic hydrolysis of lactose[5]

for reversed-phase (RP) adsorption[6]

in the hydrolysis of whey lactose[7]

Ações bioquímicas/fisiológicas

β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.[1]

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.[8]

Definição da unidade

One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.

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