Meio basal de Murashige e Skoog powder, suitable for plant cell culture, with Gamborg′s vitamins

Nível de qualidade

200

forma

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

enviado em

ambient

temperatura de armazenamento

2-8°C

Aplicação

Murashige and Skoog Basal Medium has been used:

to germinate the seeds for seedling growth and germination studies[1]

in the suspension culture of Nicotiana plumbaginifolia for transformation[2]

in the root culture of hairy clone A. artemisiifolia T4[3]

Variante da fórmula

With the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins as described by Gamborg, et al. (1968).

Media Formulation

Quantidade

Formulated to contain 4.4 grams of powder per liter of medium.

Nota de preparo

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient, micronutrient and vitamin mixtures.
Murashige and Skoog medium (M0404) contains the macro- and micronutrients of M&S basal medium as described by Gamborg, et al (1968). It can be supplemented with organics and with auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.