Tris(hidroximetil)-aminometano ACS reagent, ≥99.8%

Sinônimo(s):

2-amino-2-(hidroximetil)-1,3-propanodiol, THAM, Tris base, Trometamol

Fórmula linear:

NH₂C(CH₂OH)₃

Número CAS:

77-86-1

Peso molecular:

121.14

Beilstein:

741883

Número CE:

201-064-4

Número MDL:

MFCD00004679

ID de substância PubChem:

24855244

NACRES:

NA.25

grau

ACS reagent

Nível de qualidade

200

Ensaio

≥99.8%
99.8-100.1%

forma

crystalline

Impurezas

≤0.005% insolubles
≤2% water

useful pH range

7-9

pKa (25 °C)

8.1

pb

219-220 °C/10 mmHg (lit.)

pf

167-172 °C (lit.)

traços de cátion

Fe: ≤5 ppm
heavy metals: ≤5 ppm (ICP)

absorção

passes test

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

Descrição geral

Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma base, plays a crucial role in diverse research applications as a biological buffer. It is extensively used in the formulation of buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), owing to its optimal pKa of 8.1, making it well-suited for maintaining pH within the physiological range (pH 7 -9) applicable to most living organisms. However, caution might be necessary when studying some proteins, as Tris has the potential to interfere with the activity of certain enzymes. Tris base can be employed both independently as a buffer and as a component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, and TBE buffer, among others. Importantly, Tris base is characterized by its purity, essential stability, and relative non-hygroscopic nature.

In laboratory settings, Tris base is indispensable for the preparation of buffers compatible with biological fluids, acting as a standard pH solution. It facilitates various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it contributes to studies involving cell membrane permeability and buffer preparation.

Aplicação

O tris(hidroximetil)aminometano foi usado como solução tampão para ensaio de lactato desidrogenase, procedimento de hibridização in situ e extração de proteínas de células.[1][2][3]

Características e benefícios

• Efficient buffering within the pH range of 7-9 with a pKa of 8.1 (25 °C)

• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

• Can be used in Cell Biology, and Biochemical research

Outras notas

Compare facilmente as especificações para os produtos Trizma com a Tabela de especificações Trizma.

Informações legais

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany