Hypoxanthine - 1 Gr - Sigma
Código: 23542
Hypoxanthine powder, suitable for cell culture, BioReagent
Sinônimo(s):
6-Hydroxypurine
About This Item
Fórmula empírica (Notação de Hill):
C5H4N4O
CAS Number:
Peso molecular:
136.11
Beilstein:
5811
Número CE:
Número MDL:
Código UNSPSC:
12352207
ID de substância PubChem:
NACRES:
NA.75
Product Name
Hypoxanthine, powder, BioReagent, suitable for cell culture
linha de produto
BioReagent
Nível de qualidade
Ensaio
≥99%
Formulário
powder
técnica(s)
cell culture | mammalian: suitable
pf
>300 °C (lit.)
solubilidade
formic acid: water (2:1): 50 mg/mL
cadeia de caracteres SMILES
O=C1NC=Nc2nc[nH]c12
InChI
1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)
chave InChI
FDGQSTZJBFJUBT-UHFFFAOYSA-N
Descrição geral
Hypoxanthine (6-hydroxypurine), a purine derivative is a naturally occurring compound. It is the deaminated form of adenine and a breakdown product of adenosine monophosphate (AMP).[1]
Aplicação
Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.
Hypoxanthine has been used:
-
as a supplement in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) -buffered Roswell park memorial institute (RPMI) to culture Plasmodium falciparum strain IT/FCR3[2]
-
in the preparation of Dulbecco′s modified eagle′s medium (DMEM) + MXH for the selection of chimeric modified vaccinia virus ankara (MVA)[3]
-
to generate superoxide anions[4]
-
to prepare hybridoma selection and maintenance media.
Ações bioquímicas/fisiológicas
Hypoxanthine is capable of stimulating cell death. It can also induce reactive oxygen species (ROS). It results in endothelial dysfunction via apoptosis, stimulated by oxidative stress.[5]
