Hypoxanthine powder, suitable for cell culture, BioReagent

Sinônimo(s):

6-Hydroxypurine

About This Item

Fórmula empírica (Notação de Hill):

C₅H₄N₄O

CAS Number:

68-94-0

Peso molecular:

136.11

Beilstein:

5811

Número CE:

200-697-3

Número MDL:

MFCD00005725

Código UNSPSC:

12352207

ID de substância PubChem:

24895864

NACRES:

NA.75

Product Name

Hypoxanthine, powder, BioReagent, suitable for cell culture

linha de produto

BioReagent

Nível de qualidade

200

Ensaio

≥99%

Formulário

powder

técnica(s)

cell culture | mammalian: suitable

pf

>300 °C (lit.)

solubilidade

formic acid: water (2:1): 50 mg/mL

cadeia de caracteres SMILES

O=C1NC=Nc2nc[nH]c12

InChI

1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)

chave InChI

FDGQSTZJBFJUBT-UHFFFAOYSA-N

Descrição geral

Hypoxanthine (6-hydroxypurine), a purine derivative is a naturally occurring compound. It is the deaminated form of adenine and a breakdown product of adenosine monophosphate (AMP).[1]

Aplicação

Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.

Hypoxanthine has been used:

• as a supplement in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) -buffered Roswell park memorial institute (RPMI) to culture Plasmodium falciparum strain IT/FCR3[2]

• in the preparation of Dulbecco′s modified eagle′s medium (DMEM) + MXH for the selection of chimeric modified vaccinia virus ankara (MVA)[3]

• to generate superoxide anions[4]

• to prepare hybridoma selection and maintenance media.

Ações bioquímicas/fisiológicas

Hypoxanthine is capable of stimulating cell death. It can also induce reactive oxygen species (ROS). It results in endothelial dysfunction via apoptosis, stimulated by oxidative stress.[5]