Ethylenediaminetetraacetic acid

ACS reagent, 99.4-100.6%, powde

Sinônimo(s):

(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

About This Item

Fórmula linear:

(HO₂CCH₂)₂NCH₂CH₂N(CH₂CO₂H)₂

CAS Number:

60-00-4

Peso molecular:

292.24

Beilstein:

1716295

Número CE:

200-449-4

Número MDL:

MFCD00003541

Código UNSPSC:

12352106

eCl@ss:

39030907

ID de substância PubChem:

24894705

NACRES:

NA.77

Nível de qualidade

200

grau

ACS reagent

Agency

suitable for SM 4500 - NH3

Ensaio

99.4-100.6%

Formulário

powder

adequação da reação

reagent type: chelator

Impurezas

≤0.005% insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)

resíduo de ignição

≤0.2%

pH

2.5 (23 °C, 10 g/L)

pf

250 °C (dec.) (lit.)

solubilidade

water: soluble 0.4 g/L at 20 °C

traços de cátion

Ca: ≤0.001%
Fe: ≤0.005%
Mg: ≤5 ppm
heavy metals: ≤0.001% (byICP-OES)

cadeia de caracteres SMILES

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

chave InChI

KCXVZYZYPLLWCC-UHFFFAOYSA-N

Descrição geral

Ethylenediaminetetraacetic acid (EDTA) is a hydrophilic metal chelating agent that transforms metal ions into inactive, cyclic metal complexes. Hence, it has industrial application for resolving metal contamination.[1]

Aplicação

Ethylenediaminetetraacetic acid has been used:

• as a component of labeling buffer, which is used in the washing step during cell fractionation{155)

• for investigating the effect of papain enzyme on wound debridement and other skin issues[2]

• for harvesting rat arterial smooth muscle cell after its pre-treatment for its use in flow cytometry analysis[3]

Ações bioquímicas/fisiológicas

Ethylenediaminetetraacetic acid (EDTA) is not easily administered by the gastrointestinal tract. Intravenous administration of sodium EDTA results in hypocalcemic tetany.[4] It is generally useful in plasma or molecular diagnostics and acts by chelating calcium. EDTA serves as an anticoagulant for haematological cell counting and cell morphological analysis.[5]