Triton^™ X-100 reduced

Sinônimo(s):

Polyoxyethylene (10) isooctylcyclohexyl ether

Sobre este item

Fórmula linear:

4-(C₈H₁₇)C₆H₁₀(OCH₂CH₂)_nOH, n~10

Número CAS:

92046-34-9

UNSPSC Code:

12162002

NACRES:

NA.23

MDL number:

MFCD00167048

InChI

1S/C28H56O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h25-26,29H,6-24H2,1-5H3

SMILES string

CC(C)(C)CC(C)(C)C1CCC(CC1)OCCOCCOCCOCCOCCOCCOCCO

InChI key

QQJNBKDKLMCALZ-UHFFFAOYSA-N

refractive index

n20/D 1.473 (lit.)

density

1.029 g/mL at 25 °C (lit.)

Quality Level

200

Application

Triton^™ X-100 reduced has been used:

as a component of LB-TT for the extraction of total protein from rat brains[1]

in ADP-Glo assay and Cytophos adenosine triphosphatase (ATPase) assay[2]

in phosphate-buffered saline (PBS) solution for the permeabilization of fibroblasts in 5′ ethynyl uridine staining, immunofluorescence, and immunolabeling[3]

Features and Benefits

Non-ionic surfactant

Reduced polyoxyethylene content (~10)

Improves solubility and dispersibility of substances

Excellent wetting properties

Enhances emulsification

High purity product for research applications

General description

Triton^™ X-100 reduced (RTX-100) is formed when the benzene moiety of TX-100 is fully hydrogenated to a cyclohexane derivative. RTX-100 might enhance enzyme digestion and is also known to exhibit some effects on the photoisomerization of bacteriorhodopsin.[4]

Triton^™ X-100 reduced is a versatile nonionic surfactant with a hydrophilic polyethylene oxide chain, widely utilized as a laboratory detergent. Its applications span various areas, including cell biology and biochemical research. This detergent is commonly employed for tasks such as cell lysis to extract proteins or organelles, permeabilizing the membranes of living cells, and serving as a crucial component in lysis buffers. Triton^™ X-100 plays a key role in the isolation of lipid rafts, contributing to enhanced solubility and dispersibility of substances. With a reduced polyoxyethylene content of approximately 10, Triton^™ X-100 exhibits excellent wetting properties and facilitates emulsification.

In biochemical and cell biology research, Triton^™ X-100 is instrumental in solubilizing membrane-bound proteins and isolating lipid rafts. Its unique properties allow for the preservation of the native conformation of proteins obtained from cellular membranes in solution. Triton^™ X-100 reduced is derived from the full hydrogenation of the benzene moiety of TX-100 to a cyclohexane derivative. This modified version, RTX-100, has demonstrated potential in enhancing enzyme digestion and influencing the photoisomerization of bacteriorhodopsin, showcasing its versatility and utility in advanced research applications.

Other Notes

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Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow