Leupeptin Hydrochloride 70% (HPLC) - 100 Mg - Sigma
Código: 25047
Leupeptin hydrochloride
powder, ≥70% (HPLC)
Sinônimo(s):
Acetyl-Leu-Leu-Arg-al hydrochloride
Sobre este item
Fórmula empírica (Notação de Hill):
C20H38N6O4 · HCl
Número CAS:
Peso molecular:
463.01
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.77
MDL number:
Nome do produto
Leupeptin hydrochloride, microbial, ≥70% (HPLC)
SMILES string
Cl.CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(CCCNC(N)=N)C=O
InChI
1S/C20H38N6O4.ClH/c1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22;/h11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23);1H
InChI key
OQQYPQNFLLAILR-UHFFFAOYSA-N
biological source
microbial
assay
≥70% (HPLC)
form
powder
solubility
water: 50 mg/mL, clear, colorless to yellow
storage temp.
−20°C
Quality Level
Analysis Note
Leupeptin gives multiple peaks on HPLC because of the formation of tautomeric isomers in solution. Majority of contaminating peptide is racemized leupeptin.
Biochem/physiol Actions
Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM.
Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)
