3-Methyladenine ≥99% (HPLC), synthetic (organic), powder

Sinônimo(s):

3-MA, 6-Amino-3-methylpurine

Sobre este item

Fórmula empírica (Notação de Hill):

C₆H₇N₅

Número CAS:

5142-23-4

Peso molecular:

149.15

NACRES:

NA.51

PubChem Substance ID:

24897326

UNSPSC Code:

41106305

EC Number:

225-908-6

MDL number:

MFCD00010531

Beilstein/REAXYS Number:

146087

Assay:

≥99% (HPLC)

Biological source:

synthetic (organic)

Form:

powder

Solubility:

DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow

Storage temp.:

room temp

Nome do produto

3-Methyladenine, autophagy inhibitor

biological source

synthetic (organic)

Quality Segment

300

assay

≥99% (HPLC)

form

powder

mp

~300 °C (dec.) (lit.)

solubility

DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow

storage temp.

room temp

SMILES string

Cn1cnc(N)c2ncnc12

InChI

1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3

InChI key

FSASIHFSFGAIJM-UHFFFAOYSA-N

Application

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.

3-Methyladenine has been used as an autophagy inhibitor:

• to determine the expressions of autophagy-related genes (Beclin 1, light chain 3 (LC3) and p62) using the transfected human bone marrow mesenchymal stem cells (BM-MSCs)[1]
• with cisplatin to study the inhibition of autophagy influenced cisplatin-induced apoptosis in A2780cp cells[2]
• to study the interplay between colistin-induced autophagy and apoptosis[3]

Standard for detection of nucleic acid methylation during carcinogenesis.

Biochem/physiol Actions

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).[4]

Preparation Note

This product is soluble in DMF (10 mg/mL; may require gentle heating). It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/mL with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA may be prepared in DMSO at 100 mM.