Nisin Ready Made Solution (20,000-40,000 Iu/Ml In 0.02n Hcl) - 1 Ml - Sigma
Código: 17093
Nisin Ready Made Solution
(20,000-40,000 IU/mL in 0.02N HCl)
Synonym: Nisaplin
- CAS Number 1414-45-5
- Empirical Formula (Hill Notation) C143H230N42O37S7
- Molecular Weight 3354.07
|
Related Categories |
Antibiotic Ready Made Solutions, Antibiotics, Antibiotics A to Z, Antibiotics N-S, Biochemicals and Reagents Mais... |
|
biological source |
Lactococcus lactis |
|
form |
solution |
|
concentration |
(20,000-40,000 IU/mL in 0.02N HCl) |
|
density |
1.02 g/mL |
|
Mode of action |
cell membrane | interferes |
|
antibiotic activity spectrum |
Gram-positive bacteria |
|
shipped in |
dry ice |
|
storage temp. |
−20°C |
Descrição
Application
Due to its wide range of antimicrobial activity, nisin is used as a shelf-life extender in a broad range of dairy and non-dairy products.[1]
Biochem/physiol Actions
Nisin (Nisaplin) is produced by Lactococcus lactis. It is an effective lantibiotic peptide bactericidal agent. Nisin is a broad spectrum antimicrobial bacteriocin which affects Gram-positive bacteria, however, it has little or no effect on Gram-negative bacteria, yeasts or fungi.
It was demonstrated that nisin has capability to inhibit the growth of almost all tested Gram-positive beer-spoilage bacteria. Of 122 Gram-positive bacteria strains (predominantly Lactobacilli and Pediococci), 93% were sensitive to 100 units of nisin. In contrast, out of the 32 Gram-negative strains tested, only the genus Flavobacter proved to be affected by nisin.
Nisin exerts its antimicrobial activity by binding to lipid II which is a membrane-bound advanced intermediate involved in the biosynthesis of bacterial cell wall. By doing so, nisin sequesters lipid II from its functional location. In addition, the nisin-lipid II complex leads to formation of pores in the membrane causing cell death.
