Dipeptidyl Peptidase IV human

recombinant, expressed in Sf9 cells

Sinônimo(s):

CD26, DPPIV, Dipeptidyl aminopeptidase IV, Glycoprotein GP110

About This Item

Número CE:

3.4.14.5 (BRENDA, IUBMB)

Número MDL:

MFCD00166456

Código UNSPSC:

12352204

NACRES:

NA.54

recombinante

expressed in Sf9 cells

Nível de qualidade

200

Formulário

solution

atividade específica

≥4,000 units/μg protein

peso molecular

124 kDa

concentração

≥0.01 mg/mL

nº de adesão UniProt

P27487

doença(s) relevante(s)

cancer (lymphoma, prostate and colon cancer)

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... DPP4(1803)

Descrição geral

Dipeptidyl Peptidase IV (DPP4) is mapped to human chromosome 2q24.2.[1] It comprises the N-glycosylation sites located in the β-propeller domain.[2]

Aplicação

Dipeptidyl Peptidase IV human has been used in DPP-IV inhibitory activity assay of quinoa protein concentrate digest.[3]

Human dipeptidyl peptidase IV has been used in a study to assess the interactive hemodynamic effects of its inhibition as well as the angiotensin-converting enzyme inhibition. Human dipeptidyl peptidase IV has also been used in a study to identify and characterize the 100 kDa High Molecular Weight Hymenoptera Venom Allergens Api m 5 and Ves v 3.

Ações bioquímicas/fisiológicas

DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z. Where X is a nonspecific residue at the N terminus and Z cannot be proline or hydroxyproline.

Dipeptidyl Peptidase IV (DPP4) mediates the activation of T-cells. It is also a potential biomarker for lymphoma, thyroid, prostate and colon cancer.[1]

Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kDa. The high mannose 100 kDa DPPIV precursor is processed in the Golgi to yield a 124 kDa heavily N-and O-linked mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both N- and O-linked glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% carbohydrates, which the glycan composition is 0.9% fucose, 3.4% mannose, 5.1% galactose, 8.2% glucosamine, and 0.7% sialic acid. DPPIV is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form.

forma física

Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole and 10% glycerol.

Outras notas

One unit will hydrolyze 1.0 picomole of Ala-Pro-AMC per minute at pH 7.5 at 25 deg °C

View more information on Dipeptidyl Peptidase IV at www.sigma-aldrich.com/enzymeexplorer.