ABTS^™ Solution

solution (ready-to-use), suitable for ELISA

Sinônimo(s):

ABTS^™ Solution, ABTS

Sobre este item

Código UNSPSC:

12352204

Formulário

solution (ready-to-use)

Nível de qualidade

100

uso

sufficient for 1,500-3,000 assays (ELISA)

embalagem

pkg of 3 × 100 mL

fabricante/nome comercial

Roche

técnica(s)

ELISA: suitable

temperatura de armazenamento

2-8°C

Descrição geral

ABTS^™ Solution is a ready to use substrate for peroxidase-driven indicator reactions. It contains 2,2-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and H2O2 in glycin/citric acid buffer and is available in slightly green color.

Aplicação

ABTS Solution is a chromogenic substrate for peroxidase in ELISA assays.[1][2]

Ações bioquímicas/fisiológicas

2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) or ABTS^™ acts a substrate for HRP (horseradish peroxidase) conjugate during enzyme-linked immunosorbent assay (ELISA). It is the most sensitive, and stable substrate when compared to three other substrates namely, 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT). It also produces the best visual results, where it gives a bluish-green color. ELISA using ABTS^™ is a highly sensitive, specific and reproducible technique[3] This solution is an excellent substrate for enzyme immunoassays with horseradish peroxidase as a marker enzyme.

forma física

Reaction product:
Color: green
Evaluation: photometric (405nm)

Nota de análise

Absorption: ABTS absorption spectrum reference must always be measured and is determined by a spectrum. The Reference wavelength of 490 nm or higher should be selected due to no measureable absorption. The Reference absorption is usually automatically subtracted by the Plate Reader (background).

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações legais

ABTS is a trademark of Roche